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. Author manuscript; available in PMC: 2015 Feb 27.
Published in final edited form as: J Mol Biol. 2008 Dec 7;385(5):1397–1408. doi: 10.1016/j.jmb.2008.11.056

Figure 4.

Figure 4

Dependence of topology simplification upon circle size. (A) Degree of product simplification (R) vs DNA length (N) for relaxation by topo IV for circles of various sizes. Blue diamonds show corrected R values for small circles (see Supplementary Material). Error bars show estimates of the 95% confidence intervals of R based on multiple measurements. (B) Relaxation reaction with plasmid pQR722 (2.07 kbp) showing the final topoisomers on a gel and densitometric scan; R = 1.37.