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. Author manuscript; available in PMC: 2015 Feb 27.
Published in final edited form as: J Mol Biol. 2008 Dec 7;385(5):1397–1408. doi: 10.1016/j.jmb.2008.11.056

Figure 6.

Figure 6

DNA relaxation in the presence of a ‘roadblock’. Relaxation of plasmid pKER3 (~6.5 kbp) containing 4 EcoRI sites (blue arrows) and 7 sites (blue and black arrows). A. Plasmid map. Numbers in black inside circle indicate distances (in bp) between sites in the 4-site plasmid; numbers in blue outside circle indicate distances (in bp) between sites in the 7-site plasmid. B. The gels show relaxation by topo I and IV of the 4-site and 7-site plasmids in the presence and absence of the EcoRI mutant protein (Mut). Gels were run under the same conditions as in Figure 2.