Fig. 5.

 Expression of Hsp70 in the midgut/intestinal tissues of Lumbricus terrestris earthworms exposed in vivo to the heavy metal lead (Pb⁺⁺). Exposure time was 24 hours, and doses of Pb⁺⁺/kg of soil are indicated. The control earthworm (0 mg) was not placed in an artificial soil mixture but rather came directly from the animal stock facility. Molecular weight standards are indicated by arrows. S2 HS is Drosophila heat-shocked S2 cells; 40 μg of proteins were loaded on the gel.