Fig 3.

 Heat shock response of Bm1 RNA and Hsp70 mRNA. (a) Primer extension analysis was performed on RNA extracted from larvae using radiolabeled primers Bm Hsp70 116, Bm1 146, Bm 5S 119, and Bm1 78. RNAs are from an untreated control (lane 1) and heat shocked larvae following recovery for 0 hour (lane 2), 0.5 hour (lane 3), 1.5 hours (lane 4), 4.5 hours (lane 5), 8 hours (lane 6), 12 hours (lane 7), 18 hours (lane 8), and 24 hours (lane 9). The positions of the primer extension products and marker bands are indicated. (b) Phosphorimage analysis was performed on the 78-nt and 146-nt Bm1 RNA primer extension products from a replica experiment and those shown in panel a