Fig 7.

 Effects of interferon gamma treatment on expression of MHC-encoded Hsp70s and other proteins. HeLa cells were treated for different times as indicated up to 48 hours with interferon gamma (1000 U/mL), and samples were analyzed at 0, 12, 24, and 48 hours by Western blotting for expression of MHC class I, the constitutively expressed and interferon-inducible proteasomal subunits C3 and LMP7, and the chaperones gp96, Hsc70, Hsp70-1/2, and Hsp70-Hom. The results were normalized relative to the constitutive expression of C3 at each time point. A single-course experiment was performed, but similar results for single time points of Hsp70 levels plus and minus interferon gamma were obtained in 2 other experiments