Fig 8.

 Inducibility of MHC-encoded Hsp70s in vivo by LPS treatment. Blood samples were taken from 3 human subjects at time zero, after which each individual received LPS intravenously by injection. Blood samples were collected 2, 3, 6, 10, and 24 hours after injection and processed for white blood cell isolation and total RNA extraction. The RNA samples were amplified and cy5-dCTP–labeled cDNAs were synthesized. The fluorescent cDNAs were hybridized to a DNA microarray corresponding to human gene sequences, including hdj2, Hsp70-1/2, Hsp70-Hom, Hsp70-B, Hsc70, hdj1, and gp96, as indicated. The data points represent the average of results for 3 individual subjects, each analyzed in triplicate