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. Author manuscript; available in PMC: 2015 Feb 27.
Published in final edited form as: Cell. 2014 Oct 23;159(3):558–571. doi: 10.1016/j.cell.2014.09.049

Figure 4. Analysis of the AF9 YEATS-H3K9ac interactions.

Figure 4

(A) ITC fitting curves of AF9 YEATS titrated with different frames of the H3K9ac peptides (left) and the H3 peptides containing different modifications (right).

(B) The AF9 YEATS-H3K9ac complex structure highlighting the residues used for the mutagenesis and binding studies.

(C) ITC fitting curves of H31–10K9ac peptide with point mutants clustered to H3 binding (left) or non-binding (right) surfaces.

(D) Western blot analysis of the peptide pulldown analysis using the WT AF9 YEATS domain and the indicated point mutants.