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Immunofluorescence.
Notes: Case 1: (A) neurospheres, Musashi-1 expression; 40×; (B) id, nestin expression; 40×; (C) adherent cells, GFAP expression; 40× (confocal microscopy). Case 2 (first intervention): (D) adherent cells, GFAP expression; 40×. DAPI counterstaining was used for all.
Abbreviations: DAPI, 4′,6-diamidino-2′-phenylindole dihydrochloride; id, neurospheres.
