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Northern hybridization of Hsp70 transcripts expressed by COLO-205 (lanes 1–4), MDAMB (lanes 5–8), and PA-1 cells (lanes 9–12). Lanes 1, 5, and 9 are controls; lanes 2, 6, and 10 represent 1-hour treatment with 50 μM curcumin; lanes 3, 7, and 11 represent 2-hour treatment, and lanes 4, 8, and 12 represent 3-hour treatment with curcumin. Only 3-hour time points show expression of Hsp70 (A), PA-1 cells showing the maximum expression. (B) GAPDH control showing RNA integrity and loading
