Fig. 2.

Luciferase activity from AGC1 gene promoter constructs. Deletion analysis of the 5′-flanking region of the human AGC1 gene. The deletion fragments named C1–C6 were cloned into the pGL3 basic-LUC vector and tested for LUC expression activity in transfected SH-SY5Y cells. pGL3 indicates the pGL3 basic-LUC vector alone. Numbering indicates the extent of fragments, while gray bars indicate LUC activity. The values were set relative to the pGL3. Means ± S.D. of five duplicate independent experiments are shown. The differences compared to the pGL3 were analyzed by one-way ANOVA. *P < 0.05, ***P < 0.001.