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. 2015 Mar;60:157–166. doi: 10.1016/j.biocel.2015.01.004

Fig. 4.

Fig. 4

Downregulation of CREB in cytokine-treated SH-SY5Y cells. P-CREB, T-CREB and β-actin proteins of SH-SY5Y cells treated or untreated with 500 U/m IFNγ, 1000 U/ml TNFα and 10 ng/ml IL-1β individually or in cambination for 24 h were immunodetected with specific antibodies. P-CREB blot was reprobed for T-CREB quantification. Similar results were obtained in four independent experiments. (B) The intensities of P-CREB and T-CREB in (A) were analyzed densitometrically using a Fluor-S MultiImager and Quantity One software (Bio-Rad) and corrected for β-actin. Data are expressed as means ± S.D. of four individual experiments. *P < 0.05, **P < 0.01 versus untreated control (one-way ANOVA). (C) P-CREB, T-CREB and β-actin proteins of SH-SY5Y cells treated or untreated as above for 96 h were immunodetected with specific antibodies. P-CREB blot was reprobed for T-CREB quantification. Similar results were obtained in four independent experiments. (D) The intensities of P-CREB and T-CREB in (C) were analyzed densitometrically using a Fluor-S MultiImager and Quantity One software (Bio-Rad) and corrected for β-actin. Data are expressed as means ± S.D. of four individual experiments. *P < 0.05, **P < 0.01 versus untreated control.