Table 1. Amino acid substitutions in translated mature HA1 proteins of influenza A(H5N6) virus from Laos and H5 clade 2.3.4 reference viruses*.
| Mature HA1 position (H5 numbering)† | H5 subtype virus clade‡ |
||||||
|---|---|---|---|---|---|---|---|
| DK/LAO/XBY4 (H5N6) 2.3.4.6 | DK/GD/GD01 (H5N6) 2.3.4.6 | WD/SD/628 (H5N1) 2.3.4.6 | CK/LAO/XNY26 (H5N1) 2.3.4 | JWE/HK/1038 (H5N1) 2.3.4 | DK/LAO/3295 (H5N1) 2.3.4 | ANH/1 (H5N1) 2.3.4 | |
| 40 | R | R | K | K | K | K | K |
| 45 | N | N | N | D | D | D | D |
| 53 | K | K | K | R | R | R | R |
| 72 | R | R | R | N | N | N | N |
| 82 | R | R | R | K | K | K | K |
| 95 | L | L | L | F | F | F | F |
| 114 | T | T | I | I | I | I | I |
| 115 | L | L | L | Q | Q | Q | Q |
| 123 | P | P | P | S | S | S | S |
| 124 | N | N | D | D | D | D | D |
| 127 | T | T | T | A | A | A | A |
| 129 | L | L | L | S | S | S | S |
| 133 | A | A | A | S | S | S | S |
| 140 | M | M | A | T | T | T | T |
| 151 | T | T | I | I | I | I | I |
| 155 | D | N | D | N | N | N | N |
| 156 | A | A | A | T | K | T | T |
| 162 | M | M | I | R | R | R | R |
| 169 | R | R | R | Q | Q | Q | Q |
| 183 | N | N | N | D | D | D | D |
| 189 | N | N | N | K | K | K | K |
| 192 | K | K | K | Q | Q | Q | Q |
| 198 | V | V | V | I | I | I | I |
| 210 | E | V | V | V | V | V | V |
| 218 | Q | Q | Q | K | K | K | K |
| 223 | R | R | R | S | S | S | S |
| 240 | H | H | H | N | N | N | N |
| 263 | T | T | T | A | A | A | A |
| 265 | M | M | M | M | M | I | V |
| 269 | M | M | V | V | V | V | V |
| 273 | H | H | H | N | N | N | N |
*HA, hemagglutinin. †Sites associated with antigenicity are indicated in boldface (not comprehensive) (11). ‡Virus names: DK/LAO/XBY4, A/duck/Laos/XBY004/2014; DK/GD/GD01, A/duck/Guangdong/GD01/2014; WD/SD/628, A/wild duck/Shandong/628/2011; CK/LAO/XNY26, A/chicken/Laos/Xaythiani-26/2006; JWE/HK/1038, A/Japanese white-eye/Hong Kong/1038/2006; DK/LAO/3295, A/duck/Laos/3295/2006; ANH/1, A/Anhui/1/2005. Clade designations provided by the World Health Organization/World Organisation for Animal Health/Food and Agricultural Organization of the United Nations H5N1 Evolution Working Group unified nomenclature system for highly pathogenic avian influenza (H5N1) viruses (http://www.who.int/influenza/gisrs_laboratory/h5n1_nomenclature/en/). Proposed clade 2.3.4.6 has not been formally recognized.