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. 2015 Mar;21(3):549–550. doi: 10.3201/eid2103.141658

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Replication of porcine epidemic diarrhea virus (PEDV) in a newly established immortalized duck intestinal epithelial cell line (MK-DIEC) infected with PEDV at a multiplicity of infection of 0.1 in the presence of different concentrations of trypsin. A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate–labeled nucleoprotein-specific monoclonal antibody. B) PEDV-induced cytopathic effect in MK-DIEC cells 36 h after infection.