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. 2004 Jun 1;32(10):3005–3016. doi: 10.1093/nar/gkh623

Figure 6.

Figure 6

Putative post-translational modification of the fission yeast ZnF modules. (A) Schematic of the V5-ZnF fusion protein showing the relative location of the ZnF modules and the possible sumoylation site at Lys989 (inset: sequence surrounding K989 aligned with the consensus sumoylation site, where Ω indicates I, L or V). (B) Immunoblot using anti-V5 antibodies of total protein extracts prepared from cells carrying pREP4X (lane 1) or pREP4X-V5-ZnF (lane 2). Marker sizes are indicated (in kDa). (C) Immunoblot using anti-V5 antibodies of total protein extracts prepared from cells carrying pREP4X (lane 1), pREP4X-V5-ZnF (lane 2, labelled WT) or various ZnF1 or ZnF2 mutant pREP4X-V5-ZnF plasmids (lanes 3–12). Marker sizes are indicated (in kDa).