Table 1. Two-hybrid interactions.
| Prey | Bait | Co-expressed with | Interaction |
|---|---|---|---|
| A. Budding yeast proteins Sc Pol3 and Pol31 | |||
| – | Pol31 | – | – |
| Pol3 | – | – | – |
| Pol3 | Pol31 | – | ++ |
| Pol3-ΔZnF2 | – | – | – |
| Pol3-ΔZnF2 | Pol31 | – | – |
| Pol3-ZnF1F2 | – | – | – |
| Pol3-ZnF1F2 | Pol31 | – | +++ |
| Pol3-ZnF2 | – | – | – |
| Pol3-ZnF2 | Pol31 | – | +++ |
| B. Fission yeast proteins Sp Pol3 and Cdc1 with Cdc27 | |||
| – | Cdc1 | Cdc27 | – |
| Pol3-ZnF2 | Cdc1 | – | – |
| Pol3-ZnF2 | – | Cdc27 | – |
| Pol3-ZnF2 | Cdc1 | Cdc27 | – |
| Pol3-ZnF1F2 | Cdc1 | – | – |
| Pol3-ZnF1F2 | – | Cdc27 | – |
| Pol3-ZnF1F2 | Cdc1 | Cdc27 | +++ |
| Pol3-ZnF1F2-C1038A | Cdc1 | Cdc27 | – |
| Pol3-ZnF1F2-C1041A | Cdc1 | Cdc27 | – |
| Pol3-ZnF1F2-C1051A | Cdc1 | Cdc27 | – |
| Pol3-ZnF1F2-C1056A | Cdc1 | Cdc27 | – |
| Pol3-ZnF1F2-C1056S | Cdc1 | Cdc27 | – |
| C. Interactions with Pol ζ ZnF modules | |||
| Rev3-ZnF (Sc) | – | – | – |
| Rev3-ZnF (Sc) | Pol31 | – | – |
| Rev3-ZnF (Sp) | Cdc1 | – | – |
| Rev3-ZnF (Sp) | – | Cdc27 | – |
| Rev3-ZnF (Sp) | Cdc1 | Cdc27 | – |
Prey or bait proteins were expressed as Gal4 activation domain or LexA binding domain fusions from pACT2 or pBTM116, respectively. In A, Pol3-ZnF1F2 and Pol3-ZnF2 were identified by two-hybrid screening using a Pol31 bait (see text). Cdc27 was expressed from pAA-Cdc27. The positive interaction recorded with budding yeast Sc Pol3 and Pol31 corresponded to 15 U of β-galactosidase activity. The positive interaction recorded with fission yeast Sp Pol3 and Cdc1 corresponded to 110 U of β-galactosidase activity. All negative interactions were <2% of these values. See text for details.