Fig 5. Interaction-defective Bru mutants retain strong repressive activity in the tethering assay.

A-G, A’-G’. Egg chambers expressing the GFP-MS2 reporter mRNA. (B-G, B’-G’) also express MCP::HA₃::Bru proteins, of the type shown at left. All Bru proteins include point mutations in RRM2 and RRM3 (see Fig. 2 legend). All samples were fixed in parallel and imaged together under the same settings. Expression of the UAS transgenes was driven by the nosGAL4VP16 driver. The MCP::HA₃::Bru proteins were expressed at similar levels, except for S4E/S7E/T135E which was slightly elevated (S1B Fig.). H. RNase protection assays: GFP-MS2 RNA levels were quantified by ImageJ and normalized using the rp49 signal. The value for none, which lacks any MCP::HA₃::Bru proteins, was set to one. The mean and standard deviation were calculated from three independent experiments.