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. 2004 Jun 2;32(10):3033–3039. doi: 10.1093/nar/gkh632

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In vivo interaction of NEIL2 and p300. (A) Extracts of HCT 116 cells cotransfected with expression plasmids for FLAG-tagged NEIL2 and p300 were immunoprecipitated with anti-p300 antibody (lane 3) or preimmune sera (lane 2) and then blotted with anti-FLAG-antibody. Lane 1, FLAG-tagged NEIL2 in cell extract as marker. (B) Lanes 1 and 2, western analysis of extracts with anti-FLAG antibody. (C) Extracts of HCT 116 cells transfected with FLAG-tagged NEIL2 (lane 2) or empty vector (lane 1) were immunoprecipitated with anti-FLAG antibody and the immunoprecipitates analyzed for p300 by western blotting.