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. 2004 Jun 2;32(10):3033–3039. doi: 10.1093/nar/gkh632

Figure 2.

Figure 2

In vitro and in vivo acetylation of NEIL2. (A) In vitro acetylation of NEIL2 (lane 2) with purified p300 HAT domain and [3H]acetylCoA followed by SDS–PAGE and fluorography. Lane 1, no NEIL2. (B) Extracts of HCT 116 cells transfected with FLAG-tagged NEIL2 (lane 2) and labeled with [3H]Na-acetate were immunoprecipitated with anti-FLAG antibody and analyzed by SDS–PAGE and fluorography. Lane 1, in vitro acetylated [3H] NEIL2 marker; upper band, autoacetylated p300 HAT. (C) Western analysis with anti-AcLys antibody of anti-FLAG immunoprecipates. HCT 116 transfected with FLAG-NEIL2 (lane 2), or FLAG-NEIL2 plus p300 expression plasmids (lane 3). Lane 1, AcNEIL2 marker. (D) Western analysis with anti-FLAG antibody to compare NEIL2 levels in (C).