Table 2.

Primary binding assay data for Pinnatoxin A at 10 μM concentration

Receptor subtype	Source	3H ligand	Mean % inhibition
5-HT1A	Human (H), cloned	8-OH-DPAT	−2.5
5-HT1B	H, cloned	GR-125743 / 5CT	−5.4
5-HT1D	H, cloned	GR-125743 / 5CT	−5.9
5-HT1E	H, cloned	5HT	−0.5
5-HT2A	H, cloned	Ketanserin	0.9
5-HT2B	H, cloned	LSD	−14.5
5-HT2C	Rat, cloned	Mesulergine	38.7
5-HT3	H, cloned	LY 278,584	2.4
5-HT5A	H, cloned	LSD	−6.9
5-HT6	H, cloned	LSD	−18.3
5-HT7	H, cloned	LSD	0.0
Alpha1A	H, cloned	Prazosin / 125I-heat	27.8
Alpha1B	H, cloned	Prazosin / 125I-heat	8.7
Alpha1D	H, cloned	3H-Prazocin	4.3
Alpha2A	H, cloned	Clonidine (2nM) / 125Iodoclonidine	1.7
Alpha2B	H, cloned	Clonidine (2nM) / 125Iodoclonidine	4.0
Alpha2C	H, cloned	Clonidine (2nM) / 125Iodoclonidine	12.6
Beta1	H, cloned	Dihydroalprenolol / 125Iodopindolol	−1.8
Beta2	H, cloned	Dihydroalprenolol / 125Iodopindolol	−4.0
Beta3	H, cloned	Dihydroalprenolol / 125Iodopindolol	−7.6
BZP Rat Brain Site	Rat brain	Flunitrazepam	−0.9
Calcium Channel	Rat heart	[3H]-Nitrendipine (0.1 nM)	Ki > 10,000 nM
D1	H, cloned	SCH23390
D2	H, cloned	N-Methylspiperone, NMSP	−5.9
D3	H, cloned	N-Methylspiperone, NMSP	6.4
D4	H, cloned	N-Methylspiperone, NMSP	63.4
D5	H, cloned	SCH23390	12.9
DAT	H, cloned	WIN35428	5.9
DOR	H, cloned	DADL	10.0
GABAA	Rat, forebrain	Muscimol	39.5
H1	H, cloned	Pyrilamine	51.2
H2	H, cloned	Tiotodine	12.2
KOR	Rat, cloned	U69593	6.7
M1	H, cloned	[3H]-QNB (0.5 nM)	−11.7
M2	H, cloned	[3H]-QNB (0.5 nM)	8.5
M3	H, cloned	[3H]-QNB (0.5 nM)	27.9
M4	H, cloned	[3H]-QNB (0.5 nM)	5.0
M5	H, cloned	[3H]-QNB (0.5 nM)	−0.6
MOR	H, cloned	DAMGO	−10.3
NET	H, cloned	Nisoxetine	11.7
SERT	H, cloned	Citalopram	12.2
Sigma1	GP rat brain	[3H]-Pentazocine (3 nM)	26.6
Sigma2	PC12	[3H]-DTG (3 nM)	−14.7