Table 2. Binding of ω₂ protein to DNA studied by DNase I protection experiments.

Modified position	Nucleotide positiona	[ω2] required to reach Kd,app (in nM)
	1 2 3 4 5 6 7 1′ 2′ 3′ 4′ 5′ 6′ 7′
–	5′-…a A T C A C a a A T C A C a …-3′	∼20
1	5′-…C —————→ ——————→…-3′	∼10
1	5′-…G—————→ ——————→…-3′	∼20
2	5′-… —T ————→ ——————→…-3′	∼20
2	5′-… —C ————→ ——————→…-3′	>160
2	5′-… —G ————→ ——————→…-3′	∼20
3	5′-…——A———→ ——————→…-3′	∼80
3	5′-…——C———→ ——————→…-3′	∼80
3	5′-…——G———→ ——————→…-3′	∼160
4	5′-…———T ——→ ——————→…-3′	∼80
4	5′-…———A ——→ ——————→…-3′	∼160
4	5′-…———G ——→ ——————→…-3′	>160
5	5′-… ————T —→ ——————→…-3′	>160
5	5′-… ————C —→ ——————→…-3′	∼160
5	5′-… ————G —→ ——————→…-3′	∼160
6	5′-… —————T→ ——————→…-3′	>160
6	5′-… —————A→ ——————→…-3′	>160
6	5′-… —————G→ ——————→…-3′	>160
7	5′-… ——————C ——————→…-3′	∼10
7	5′-… ——————G ——————→…-3′	∼20

The DNA contained two directly repeated heptads of which the upstream one was mutated by one base in consecutive positions as shown on each line.

^aThe heptad sequences are flanked by three adenines at the 5′-ends and three cytosines at the 3′-ends and surrounded by the multi-cloning site of the vector. The base pair difference with wt is highlighted.