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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Dec 30;35(3):570–572. doi: 10.1161/ATVBAHA.114.304938

Figure 1.

Figure 1

Characterization of HDL using Superose 6 chromatography and density gradient subfractionation. (A) Protein and lipid contents of the Superose 6 column fractions. (B) Anticoagulant and procoagulant profiles for Superose 6 column fractions. (C) Loss of anticoagulant activity over time for the HDL- containing fractions. (D) Anticoagulant activity of HDL subfractions following density gradient subfractionation. Dotted vertical line indicates density of 1.125 g/mL and solid vertical line a density of 1.21 g/mL. Enhancement of APC:Protein S anticoagulant activity is reflected in the “Normalized Ratio” which was calculated as the ratio of prothrombin times plus and minus APC:Protein S normalized to 1.0 for samples lacking Superose 6 fractions.