Figure 2.

Sensorgrams of LNA/DNA R06 derivative–miniTAR complexes. A total of 500 nM concentration of LNA5 (thin line), LNA4 (bold line) or anti-loop LNA5 (dotted line) were injected on a miniTAR-functionalized sensorchip. Rate constants, k_on and k_off, for bimolecular complex formation were deduced from direct fitting of these plots according to equations 1 and 2 (Materials and Methods). Three independent experiments were carried out in R buffer (3 mM Mg²⁺) at 23°C.