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. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: J Cell Physiol. 2015 Jun;230(6):1189–1198. doi: 10.1002/jcp.24814

Effect of calcitriol and Vitamin D₃ on TNF-α-induced MMP-2 and MMP-9 activity. Fetal ASM were treated with vehicle, (A, B) calcitriol, or (C) Vitamin D₃, followed by media only or TNFα. MMP-2 and MMP-9 activity in cell culture media was determined by zymography. Calcitriol blunted TNFα-induced increase in MMP-9 activity, but had no effect on MMP-2. Furthermore, the inactive Vitamin D₃ was without effect on MMP-9 activity. Gels are representative results from independent experiments using at least 3 individual samples. For summary bar graphs, pro-and cleaved MMP data were quantified together and normalized to vehicle control. Data are presented as mean ± SEM, n=4 samples, p<0.05. * indicates significant difference from vehicle control; ^$ indicates significant difference from TNFα.

Effect of calcitriol and Vitamin D₃ on TNF-α-induced MMP-2 and MMP-9 activity. Fetal ASM were treated with vehicle, (A, B) calcitriol, or (C) Vitamin D₃, followed by media only or TNFα. MMP-2 and MMP-9 activity in cell culture media was determined by zymography. Calcitriol blunted TNFα-induced increase in MMP-9 activity, but had no effect on MMP-2. Furthermore, the inactive Vitamin D₃ was without effect on MMP-9 activity. Gels are representative results from independent experiments using at least 3 individual samples. For summary bar graphs, pro-and cleaved MMP data were quantified together and normalized to vehicle control. Data are presented as mean ± SEM, n=4 samples, p<0.05. * indicates significant difference from vehicle control; ^$ indicates significant difference from TNFα.