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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Cell Physiol. 2015 Jun;230(6):1189–1198. doi: 10.1002/jcp.24814

Figure 5.

Figure 5

Effect of calcitriol on ECM deposition. (A) collagen I, (B) collagen III, and (C) fibronectin deposition by fetal ASM was evaluated by In-Cell Western (representative examples of wells are shown in panel D), with normalization to control. Both TNFα and TGFβ increased ECM deposition, and calcitriol blunted these effects, albeit not for every ECM protein and cytokine. Wells are representative results from independent experiments using at least 3 individual samples. Data are presented as mean ± SEM, n=4 samples, p<0.05. * indicates significant difference from vehicle control; $ indicates significant difference from TNFα; # indicates significant difference from TGFβ.