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. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: J Cell Physiol. 2015 Jun;230(6):1189–1198. doi: 10.1002/jcp.24814

Role of Vitamin D receptor (VDR) in calcitriol effects in fetal ASM. (A) Fetal ASM cells expressed VDR mRNA, which was not substantially altered by exposure to calcitriol or TNF-α. (B) Western analysis showed that VDR protein levels were maintained following calcitriol or TNFα treatment. (C) siRNA knockdown of VDR (verified by Western analysis (B)) blunted calcitriol effects on MMP-9 activity in the presence of TNFα, as assessed by zymography. Western blots are representative results from independent experiments using at least 3 individual samples. Data are presented as mean ± SEM, n=at least 3 samples, p<0.05. * indicates significant difference from vehicle control; ^$ indicates significant difference from TNFα.

Role of Vitamin D receptor (VDR) in calcitriol effects in fetal ASM. (A) Fetal ASM cells expressed VDR mRNA, which was not substantially altered by exposure to calcitriol or TNF-α. (B) Western analysis showed that VDR protein levels were maintained following calcitriol or TNFα treatment. (C) siRNA knockdown of VDR (verified by Western analysis (B)) blunted calcitriol effects on MMP-9 activity in the presence of TNFα, as assessed by zymography. Western blots are representative results from independent experiments using at least 3 individual samples. Data are presented as mean ± SEM, n=at least 3 samples, p<0.05. * indicates significant difference from vehicle control; ^$ indicates significant difference from TNFα.