Fig. 5.

Donor T cells fail to persist in anti-CD40-conditioned SV11 mice following acute tumor regression. Groups of SV11 mice received either anti-CD40, control IgG, or WBI conditioning prior to ACT with CD90.1⁺ TCR-IV T cells. On day +30, cells from a spleens, cLN (not shown), and b brains were stained for CD90.1 and CD8. Values on dot plots indicate percent CD90.1⁺ of total CD8⁺ cells (mean ± SEM). c Total CD90.1⁺ cells in spleens, cLN, and brains on day +30 are plotted. Representative histograms of CD44, CD62L, and KLRG1 expression gated on live CD45.2⁺CD8⁺CD90.1⁺ TCR-IV T cells (open histogram) or CD45.2⁺CD8⁺CD90.1⁻ T cells (filled histogram, spleen only) are shown on day +30 in d spleen and e brain. Values indicate the percent of TCR-IV T cells within the indicated gate (mean ± SEM). Samples with <25 tetramer-IV⁺ T cells were not included in phenotype analyses. Data are representative of 3–5 mice/group. Statistical significance was determined using one-way ANOVA with Bonferroni posttest. **p < 0.01; ***p < 0.001; ****p < 0.0001; ns not significant