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. Author manuscript; available in PMC: 2015 May 23.
Published in final edited form as: Oncogene. 2014 Jun 16;34(17):2189–2203. doi: 10.1038/onc.2014.175

Figure 6. RECK depletion elevates VEGF and uPA secretion, activation of a coordinate pro-angiogenic program, and increases invasive potential but does not affect cell growth.

Figure 6

(A) Representative immunoblot of Hs343T and Hs606T cells 48h after transfection with SCR (scramble) or RECK siRNA.

(B) Growth curve of Hs606t cells transfected with indicated siRNA. Data is presented as mean ± SEM (ns=not significant, n=3).

(C) VEGF ELISA on conditioned media isolated from Hs343T and Hs606T transfected with indicated siRNA. Data is presented as mean ± SEM (***p<0.001, n=3).

(D) qRT-PCR of VEGF gene expression after transfection with indicated siRNA. Data is presented as mean ± SEM (**p<0.01, n=3).

(E) uPA ELISA on conditioned media isolated from Hs343T and Hs606T transfected with indicated siRNA. Data is presented as mean ± SEM (***p<0.001, n=3).

(F) qRT-PCR of PLAU gene expression after transfection with indicated siRNA. Data is presented as mean ± SEM (**p<0.01, n=3).

Quantification of Boyden transwell invasion of (G) Hs343T and (H) Hs606T cells transfected with indicated siRNAs. Data is presented as mean ± SEM (***p<0.001, n≥3).

Conditioned media from Hs606T cells transfected with indicated siRNA was used to probe an angiogenesis antibody array. (I) Image of array was used to quantify protein expression by densitometry, and altered candidates are shown in heat map (J).