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. 2004 Jun 10;32(10):3148–3155. doi: 10.1093/nar/gkh638

Figure 3.

Figure 3

DMS protection and interference analysis of KpnI REase. The guanine-specific contacts of KpnI REase were obtained by DMS protection (A) and interference analysis (B). Both top strand (Top) and bottom strand (Bot) interactions were probed as described in Materials and Methods. F and C refer to the methylation pattern in the absence and presence of the enzyme, respectively. The guanine contacts in the recognition sequence are indicated and summarized in the nucleotide sequence given below.