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. 2013 Sep 17;19(1):294–302. doi: 10.2119/molmed.2013.00069

Figure 2.

Figure 2

Fluorescence-activated cell sorting and phenotypic characterization of MZ, FM and GC B cells. (A) Plots shown are gated on CD19+ B cells. Staining for sIgD/CD38 expression enables the separation of FM, GC and IgDCD38 B cells. The latter cells are further separated into MZ and SM B cells as indicated. Numbers indicate the percentage of cells in the boxes. (B) Cell surface marker analysis of the purified B-cell subsets. (C) Staining for sIgG or sIgA of SM B cells. The gray area represents the negative control staining with an unrelated mAb. Red, green, blue and purple line colors correspond to MZ, GC, FM and SM, respectively.