Table 1. Oligonucleotides used in this study.
Oligo | Sequence |
---|---|
1 | d(TTTTTTTTTTTTTTTTTTTTTGGCGACGGCAGCGAGGC) |
2 | d(GCCTCGCTGCCGTCGCCA) |
3 | d(TGGCGACGGCAGCGAGGCTTTTTTTTTTTTTTTTTTTT) |
4 | d(ACGTATCGTAGATATACTATGTATTGGCAGAGTGGACAATT) |
5 | d(AATTGTCCACTCTGCCAATACATAGTATATCTACGATACGT) |
6 | d(GCGATAGTCTCTAGACAGGTACAGGATCGTTACTAATCGTCTATGACGTG) |
7 | d(GCCTCGCTGCCGTCGCCTTTTTTTTTTTTTTTTTTTTT) |
8 | d(CACGTCATAGACGATTACATTGCTAGGACATGCTGTCTAGAGACTATCGC) |
9 | d(GATCCAACCGGCTACTCTAATAGCCGGTTGGACGCACATACTGTGTGCATATG)a |
10 | d(CATATGCACACAGTATGTGCGTCCAG) |
11 | d(GATCCAATCGGCCATTAGAGTGGCCGATTGGACGCACATACTGTGTGCATATG) |
12 | d(GATCCAATCGGCCATGGCCGATTGGACGCACATACTGTGTGCATATG) |
13 | d(CATATGCACACAGTATGTGCGTCCA) |
14 | d(ACCGGCTACTCTAATAGCCGGTTGGACGCACATACTGTGTGCATATG) |
15 | d(ATATGCACACAGTATGTGCGTCC) |
16 | d(AACCGGCTACTCTAATAGCCGGTTGGACGCACATACTGTGTGCATAT) |
17 | d(TATGCACACAGTATGTGCGTCCAACCGGCTATTAGAGTAGCCGGTTTGATCC) |
18 | d(GTGGACGCACATACTGTGTGCATATGGATC) |
19 | d(CATATGCACACAGTATGTGCGTCCAG) |
20 | d(GATCCATATGCACACAGTATGTGCGTCCAACCGGCTATTAGAGTAGCCGGT) |
aThe underlined sequences can basepair and form folded structures. The HpaII cleavage sequence is present within this region and cleavage with this enzyme was used to confirm the presence of basepaired regions in these oligonucleotides.