FIG. 2.

(a) Schematic of the optical path through the epi-fluorescence optical hub. The “blue” channel emits LED light centered around 490 nm for standard FITC and EGFP illumination. The “amber” channel emits LED light centered around 575 nm and provides rhodamine and Texas red illumination. (b) Technical drawing showing the retrofitted objective (rotated 90°). The liquid lens, shaded in purple, sits between custom-built brass pieces (orange), one of which makes electrical contact with the contact pin (yellow) while the other serves as case ground. The insulating ring (green) isolates the applied voltage from ground. (c) Epi-fluorescence optical hubs that direct excitation light for both illumination modes in all 12-channels of the AHT.