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. 2004 Jun 15;32(10):3212–3219. doi: 10.1093/nar/gkh643

Figure 3.

Figure 3

(a) Mg2+ dependence of the inducible construct iHP–TRAP in the presence of 50 nM trp-mRNA. (b) Spermidine dependence, with increasing Mg2+ concentrations, of the inducible construct iHP–TRAP in the presence of 50 nM trp-mRNA, in FBB and 1.0 mM l-tryptophan at 37°C. Notably, at a concentration of 8.0 mM spermidine iHP–TRAP shows activity in the presence of 50 nM mRNA despite the low concentration of Mg2+ (4.0 mM).