Figure 4.
Summary of the influence of AUG recognition on cleavage of 18S rRNA residues in the PIC. The locations of 18S rRNA cleavages are depicted in the yp48S PIC (14), with tRNAi, mRNA and 18S rRNA colored as in Figure 2. 18S rRNA residues in blue displayed AUC>AUG differential cleavage; blue residues with side chains depicted as sticks also showed diminished AUC>AUG cleavage resulting from suppression of cleavage in mRNA(AUC) complexes by the K11C/SE* variant compared to that seen for the K11C variant; while blue residues depicted as spheres also displayed enhanced cleavage in mRNA(AUG) complexes by K11C/SE* and V129C/SE* compared to that given by the K11C and V129C variants, respectively. Gold residues were cleaved constitutively; while cyan residues exhibited enhanced cleavage in AUG versus AUC complexes specifically by the Q73C variant.