Figure 6.

Growth, polysome and rRNA processing analysis for Δsnr40. (A) Ten-fold serial dilutions of the strains were spotted onto solid YPD plates and were incubated at different temperatures. (B) Polysome profile of isogenic wild type (B) and Δsnr40 (C). (D) Illustration for the 35S primary transcript. 35S rRNA contains 18S, 5.8S and 25S rRNA sequences separated by ITS1 and ITS2. Processing of 35S rRNA to mature rRNA involves endonucleolytic and exonucleolytic steps at specific sites. (E) Ethidium bromide (EtBr) stained gel-carrying mature 25S and 18S rRNA from wild type, Δsnr40 and Δsnr40 + pJN22 strains separated on 1% agarose gel along with Northern blot analysis of the rRNA processing in WT, Δsnr40 and Δsnr40+pJN22. The membrane was hybridized with radioactive (³²P) labeled probe 5 for ITS1. (F) Paromomycin sensitivity test was performed by spotting 5 μl paromomycin solution on filter discs, which were then applied on YPD plates containing Δsnr40 strain.