Figure 2.

Phosphodiester linkage and sugar heterogeneity has distinct impacts on pol II transcription efficiency. Pol II catalytic rate constants (k_pol) (a), substrate dissociation constants (b), relative efficiency of pol II nucleotide incorporation (c) were shown for pol II complex with four template backbone variants. The efficiency was normalized according to the specificity constants (k_pol/K_d,app) of pol II transcription through these four templates. (d) Comparisons of phosphodiester linkage and sugar heterogeneity effects on pol II incorporation. The values are calculated based on the specificity constants (k_pol/K_d,app).