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. 2015 Feb 4;43(4):2022–2032. doi: 10.1093/nar/gkv063

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Telomere elongation suppresses the up-regulation of innate immune gene expression in in vivo tumors. (A) Retroviral overexpression of hTERT (upper panels) and up-regulation of telomerase activity (middle panel) at PD 50. Size markers are indicated on the right. After the introduction of Cre at PD 40, only endogenous hTERT and telomerase activities were detected in PC-3/LhTERTL/cre+ cells. Bottom panel: telomere Southern blot analysis at PD 77. PC-3/LhTERTL, HBC4/hTERT and MKN74/hTERT cells show elongated telomeres. PC-3/LhTERTL/cre+ cells also maintained long telomeres even after shutdown of the exogenous hTERT gene at PD 40. (B) Expression of STAT1, ISG15, OAS3 and GAPDH mRNA in telomere-elongated cancer cells in 2D dishes (2D) and xenografts (xeno) were analyzed by qPCR and normalized to GUSB mRNA levels. Error bars indicate standard deviations (n = 3).