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. 2015 Feb 6;43(4):2353–2366. doi: 10.1093/nar/gkv070

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Interaction of Luzp4 with mRNA export factors. (A) Schematic of Luzp4 putative domains organisation. The UBM previously identified in Alyref and Uif is aligned with the homologous sequence identified in Luzp4. Residues in bold show strict identity to Alyref sequence. Black dots show biochemically conserved positions. Arginine-Serine (RS) dipeptides are indicated as vertical grey bars. The alternative names used for Luzp4 are also shown. (B) GST pulldown assays with the indicated proteins. (C) Co-IP assays using the indicated expression vectors. Western blots were probed with the indicated antibodies. (D) GST-pulldown analysis of ³⁵S labelled Luzp4 truncations as indicated with either GST-Nxf1:p15 (right panel) or GST-Uap56 (lower panel). The ³⁵S labelled protein inputs are shown in the left panel. In each case the panel shown is a phosphoimage. Corresponding Coomassie-stained gels are presented in Supplementary Figure S2B. (E) Schematic of the truncation constructs used to map binding sites for Nxf1 and Uap56 and summary of the binding data.