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. 2015 Feb 6;43(4):2353–2366. doi: 10.1093/nar/gkv070

Figure 6.

Figure 6.

Luzp4 is expressed in cancer cells and involved in MeWo cell growth. (A) Summary of the qRT-PCR analysis of Luzp4, Uif and Alyref expression in RNA samples derived from 402 different patients, representing multiple different clinical stages of tumours from many tissues. The full data set for each tissue is shown in Supplementary Figure S7. Genes were defined as expressed when 2−ΔCT >0.04. (B) qRT-PCR analysis of expression Alyref, Uif and Luzp4 in MeWo and 293T cells. Values for LUZP4 levels in 293T and MeWo were 0.00055 and 0.06433, respectively. The results represent the average of three independent experiments. The error bars represent the standard deviation. (C) qRT-PCR analysis to confirm efficient knockdown of ALYREF, LUZP4 and UIF mRNA in MeWo cells using siRNAs. The values shown are normalized to U1 snRNA abundance. The error bars represent the standard deviation from three independent experiments. (D) Colony formation assay for MeWo cells following knockdown of the indicated mRNAs using siRNAs. The values shown for all graphs represent the average of five independent experiments, each containing six replicates per condition and the error bars represent the standard deviation. **P < 0.01, ***P < 0.001, ****P < 0.0001.