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Wild-type or mutant Polη displays the same catalytic activities. Time course of DNA synthesis catalyzed by wild-type (WT) or mutant Polη using a primed single-stranded template (pUC118.ss). Upper panel: DNA products were subjected to electrophoresis on a 20% polyacrylamide–7-M urea denaturing gel. Lower panel: immunoblot of the different forms of Polη produced in rabbit reticulocytes lysates, using an anti-myc antibody.
