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. 2015 Feb 4;43(4):2138–2151. doi: 10.1093/nar/gkv082

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Disabling the DNA damage response suppressed the requirement of Ku for proliferation of U. maydis cells. (A) Serial 10-fold dilutions of FB1 (WT), UMP122 (chk1Δ), UCS1 (atr1Δ), UCS33 (uku70^nar1), UCS35 (uku70^nar1 chk1Δ), UCS40 (uku70^nar1 atr1Δ), UCS30 (uku80^nar1), UCS39 (uku80^nar1 chk1Δ) and UCS44 (uku80^nar1 atr1Δ) cultures were spotted on solid rich medium (YPD) and minimal medium with nitrate (MMD). YPD plates were incubated for 2 days and the nitrate plates for 4 days at 28°C. (B) Morphology of uku70^nar1 (UCS33), uku80^nar1 (UCS30), uku70^nar1 chk1Δ (UCS35), uku70^nar1 atr1Δ (UCS40), uku80^nar1 chk1Δ (UCS39) and uku80^nar1 atr1Δ (UCS44) cells incubated for 8 h in restrictive conditions (YPD). All cells were shown at the same magnification, Bar: 15 μm. (C) FACS analysis of FB1 (WT), UCS33 (uku70^nar1), UCS35 (uku70^nar1 chk1Δ), UCS40 (uku70^nar1 atr1Δ), UCS30 (uku80^nar1), UCS39 (uku80^nar1 chk1Δ) and UCS44 (uku80^nar1 atr1Δ) after 8 h of incubation in permissive (minimal medium with nitrate, MMD) or restrictive conditions (rich medium, YPD). 1C and 2C indicate haploid and diploid DNA content.