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. 2015 Feb 4;43(4):2138–2151. doi: 10.1093/nar/gkv082

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Disabling the DNA damage response does not suppress the formation of altered telomeres. (A) DNAs from the indicated strains (FB1 (WT), UCS33 (uku70^nar1), UCS35 (uku70^nar1 chk1Δ), UCS40 (uku70^nar1 atr1Δ), UCS30 (uku80^nar1), UCS39 (uku80^nar1 chk1Δ), UCS44 (uku80^nar1 atr1Δ), UMP122 (chk1Δ) and UCS1 (atr1Δ)) grown in restrictive (−, YPD) or permissive (+, MMD) conditions for 18 h were isolated, digested with PstI and hybridized with a radioactively labeled telomere-specific probe. (B) DNAs from the indicated strains (FB1 (WT), UCS33 (uku70^nar1), UMP218 (uku70^nar1 mre11Δ), UMP235 (uku70^nar1 mre11Δ/mre11) and UMP236 (uku70^nar1 mre11Δ/mre11^H228N) grown in restrictive (−, YPD) or permissive (+, MMD) conditions for 18 h were isolated, digested with PstI and hybridized with a radioactively labeled telomere-specific probe. (C) In vivo phosphorylation of Chk1. UMP124 (wt), UMP231 (uku70^nar1), UMP228 (uku70^nar1 mre11Δ), UMP237 (uku70^nar1 mre11Δ/mre11) and UMP238 cells carrying an endogenous Chk1-3MYC allele were incubated in YPD (restrictive conditions) for 8 h. Protein extracts were immunoprecipitated with a commercial anti-MYC antibody and the immunoprecipitates were subjected to SDS-PAGE and immunoblotted with the anti-MYC antibody. (D) Serial 10-fold dilutions of FB1 (WT), UCS33 (uku70^nar1), UMP218 (uku70^nar1 mre11Δ), UMP235 (uku70^nar1 mre11Δ/mre11) and UMP236 (uku70^nar1 mre11Δ/mre11^H228N) cultures were applied to solid rich medium (YPD) and minimal medium with nitrate (MMD). YPD plates were incubated for 2 days and the nitrate plates for 3 days at 28°C.