Figure 7.
Mre11 forms foci at telomeres upon Ku depletion. (A) Live cell analysis of Mre11-GFP and its co-localization with Pot1-cherry. UMP230 (wt), UMP231 (uku70nar1) and UMP234 (uku70nar1 chk1Δ) cells were incubated for 8 h in restrictive conditions (YPD) and images were taken with the corresponding set of filters. Pictures showed DIC and merged images from GFP and cherry fluorescence of representative samples. Each inset shows a magnified image of a nucleus. Bar: 15 μm. (B) Quantification of the percentages of cells displaying co-localized Mre11 and Pot1 foci.