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Conditional and targeted expression of EndoIII and EndoVIII into mitochondria. Western blots containing 20 μg of mitochondrial extracts isolated from (A) Dox ± vector and clone 3 of HeLa Tet-On/MTS–EndoIII cells; and (B) Dox ± vector and three separate clones (2, 3 and 4) of HeLa Tet-On/MTS–EndoVIII transfected cells using anti-HA antibodies. Immunodetection of cytochrome c was performed to show that the recombinant proteins are in mitochondria.
