Figure 3.

Dox-regulated EndoIII and EndoVIII activities in mitochondria. Enzyme activity was measured using a labeled 24 mer containing Tg. (A) After OsO₄ treatment, 5′ ³²P end-labeling and annealing to the complementary oligonucleotide, the Tg-containing duplex was digested with EndoIII. Mitochondrial fractions were isolated from vector (Dox−) (white solid bar), (Dox+) (white dashed bar) and (B) either MTS–EndoIII (Dox−) (black solid bar), (Dox+) (black dashed bar) or (C) MTS–EndoVIII (Dox−) (gray solid bar), Dox+ (gray dashed bar) clones and the extracts incubated with labeled substrate. The data represent three independent experiments. An asterisk indicates a significant difference (p < 0.01).