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. 2004 Jun 15;32(10):3240–3247. doi: 10.1093/nar/gkh648

Figure 4.

Figure 4

Conditional Expression of EndoIII and EndoVIII in mitochondria and mtDNA repair. MTS–EndoIII, MTS–EndoVIII and vector transfectants were treated with 800 μM menadione for 20 min and lysed immediately or allowed to repair for 2 or 6 h in Dox ± media. Control cells were exposed to the drug diluent only. Total DNA was isolated from the lysed cells and quantitative Southern blot analysis with mtDNA specific probe performed. (A) Representative autoradiographs for each cell type are displayed. (B) The percentage of repair for MTS–EndoIII clones (Dox−) (open circles), (Dox+) (solid circles). (C) The percentage of repair for MTS–EndoVIII transfectants (Dox−) (open triangles), Dox+ (filled triangles). The percentages of repair for vector transfectants (Dox-) (open squares), Dox+ (filled squares) are shown in both (B) and (C).The data are the means ± SE of three separate experiments. An asterisk indicates a significant difference (p < 0.01).