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. 2015 Feb 8;43(4):2164–2176. doi: 10.1093/nar/gkv092

Figure 2.

Figure 2.

The DNA-binding activity of the CpCdc13AB complex. (A) CpCdc13A, Cdc13B and the Cdc13AB complex (∼30 nM) purified by affinity chromatography and glycerol gradients were tested for binding to the CpG2 probe (7.5 nM). Three different concentrations of unlabeled CpG2 oligos (at 3×, 9× and 30× the probe concentration) were added to the assays in order to judge the specificity of the interaction. (B) Left: Gel mobility shift assays were performed using the CpG2 probe (7.5 nM) and the AB complex (10 nM). Increasing concentrations of unlabeled telomeric oligos (at 23, 75 and 225 nM) were added to the assays as competitors. Cp, Cl, Ct and Sc are abbreviations for C. parapsilosis, C. lusitaniae, C. tropicalis and S. cerevisiae. Right: The levels of the complex in the assays were normalized against that in the absence of competitor and plotted against the competitor/probe ratios.