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. 2015 Feb 8;43(4):2164–2176. doi: 10.1093/nar/gkv092

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Combinatorial recognition of telomere G-strand by the CpCdc13AB dimer. (A) The oligonucleotides used for the site-specific crosslinking analysis are illustrated. The positions of the 5-Iodo-2’-deoxyuridine analog are indicated by asterisks. (B) The CpCdc13AB dimer was incubated with the indicated oligonucleotides (pre-labeled with P³²) and subjected to UV irradiation. The covalent protein–DNA conjugates were separated from free DNA by SDS-PAGE and detected by PhosphorImager analysis. The specificity of the crosslinking reaction was tested by adding 100-fold excess of unlabeled CpG1 competitor to the reactions. (C) Left: The CpCdc13AB dimer was subjected to Ulp1 treatment to eliminate the SUMO tag from the Cdc13A fusion protein. Right: Untreated or Ulp1-treated CpCdc13AB dimer was subjected to crosslinking assays using the indicated substrates.