Figure 5.

Introduction of antibodies directed against proteins in the RMN complex sensitizes normal diploid fibroblasts to agents that damage chromosomal DNA. (A) Cell death was determined following treatment with 20 U of the restriction endonuclease PvuII in cells co-electroporated with non-specific IgG (1), anti-Rad50 antibody (2), anti-Mre11 antibody (3), anti-Nbs1 antibody (4), both anti-Rad50 and anti-Mre11 antibodies (5), both anti-Rad50 and anti-Nbs1 antibodies (6), both anti-Mre11 and anti-Nbs1 antibodies (7), and anti-Rad50, anti-Mre11 and anti-Nbs1 antibodies (8). (B) Cell death was determined after treatment with 200 ng/ml diepoxybutane of cells electroporated with the same antibodies as in (A).