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. 2015 Jan 23;7(2):799–814. doi: 10.3390/nu7020799

Figure 4.

Figure 4

Effects of protopanaxadiol (PPD) on 5-FU-induced apoptosis. HCT-116 human colorectal cancer cells were treated with PPD at various concentrations (10 and 20 µM) in the absence or presence of 5-FU (30 µM) for 48 h. Apoptosis was quantified using annexin V/PI staining followed by flow cytometric analysis. (A) Representative scatter plots of PI (y-axis) vs. annexin V (x-axis) in each experimental group; (B) the percentage of apoptotic cells. Data obtained from triplicate experiments. + p < 0.05; ++ p < 0.01 compared to control; ** p < 0.01 compared to 5-FU group.