Figure 5.

Signal variation comparisons for features with optimal RNA denominator signals. (A) Cy5-labeled C2C12 cDNA was hybridized against Cy3-labeled Stratagene mixed cDNAs in five replicate experiments. Features with denominator signals >200 and <10 000 in all five Stratagene replicates were extracted [n = 6190/13 916 features (44%)]. Log₂ CV (%) for all 6190 features are plotted in the histogram. (B) The list of features analyzed in (A) was used to extract corresponding values from five replicate experiments in which Cy5-labeled C2C12 cDNA was cohybridized against Cy3-labeled genomic DNA. Log₂ CV (%) for all 6190 features are plotted.